|
KMID : 0624620100430030193
|
|
BMB Reports
2010 Volume.43 No. 3 p.193 ~ p.198
|
|
|
ZNF552, a novel human KRAB/C2H2 zinc finger protein, inhibits AP-1- and SRE-mediated transcriptional activity
|
|
Deng Yun
Liu Bisheng
Fan Xiong-Wei
Wang Yue-Qun
Tang Ming
Mo Xiao-Yan
Li Yong-Qing
Ying Zaochu
Wan Yongqi
Luo Na
Zhou Jun-Mei
Wu Xiu-Shan
Yuan Wu-Zhou
|
|
|
Abstract
|
|
|
|
In this study, we report the identification and characterization of a novel C2H2 zinc finger protein, ZNF552, from a human embryonic heart cDNA library. ZNF552 is composed of three exons and two introns and maps to chromosome 19q13.43. The cDNA of ZNF552 is 2.3 kb, encoding 407 amino acids with an amino-terminal KRAB domain and seven carboxyl-terminal C2H2 zinc finger motifs in the nucleus and cytoplasm. Northern blotting analysis indicated that a 2.3 kb transcript specific for ZNF552 was expressed in liver, lung, spleen, testis and kidney, especially with a higher level in the lung and testis in human adult tissues. Reporter gene assays showed that ZNF552 was a transcriptional repressor, and overexpression of ZNF552 in the COS-7 cells inhibited the transcriptional activities of AP-1 and SRE, which could be relieved through RNAi analysis. Deletion studies showed that the KRAB domain of ZNF552 may be involved in this inhibition.
|
|
|
KEYWORD
|
|
|
C2H2, KRAB, RNAi, Signal pathway, ZNF552
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|